This example explains how to register 2D images using the Symmetric Normalization (SyN) algorithm proposed by Avants et al. [Avants09] (also implemented in the ANTs software [Avants11])

We will perform the classic Circle-To-C experiment for diffeomorphic registration

```
import numpy as np
from dipy.data import get_fnames
from dipy.align.imwarp import SymmetricDiffeomorphicRegistration
from dipy.align.metrics import SSDMetric, CCMetric, EMMetric
import dipy.align.imwarp as imwarp
from dipy.viz import regtools
fname_moving = get_fnames('reg_o')
fname_static = get_fnames('reg_c')
moving = np.load(fname_moving)
static = np.load(fname_static)
```

To visually check the overlap of the static image with the transformed moving image, we can plot them on top of each other with different channels to see where the differences are located

```
regtools.overlay_images(static, moving, 'Static', 'Overlay', 'Moving', 'input_images.png')
```

We want to find an invertible map that transforms the moving image (circle) into the static image (the C letter).

The first decision we need to make is what similarity metric is appropriate for our problem. In this example we are using two binary images, so the Sum of Squared Differences (SSD) is a good choice.

```
dim = static.ndim
metric = SSDMetric(dim)
```

Now we define an instance of the registration class. The SyN algorithm uses a multi-resolution approach by building a Gaussian Pyramid. We instruct the registration instance to perform at most \([n_0, n_1, ..., n_k]\) iterations at each level of the pyramid. The 0-th level corresponds to the finest resolution.

```
level_iters = [200, 100, 50, 25]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters, inv_iter = 50)
```

Now we execute the optimization, which returns a DiffeomorphicMap object, that can be used to register images back and forth between the static and moving domains

```
mapping = sdr.optimize(static, moving)
```

It is a good idea to visualize the resulting deformation map to make sure the result is reasonable (at least, visually)

```
regtools.plot_2d_diffeomorphic_map(mapping, 10, 'diffeomorphic_map.png')
```

Now let’s warp the moving image and see if it gets similar to the static image

```
warped_moving = mapping.transform(moving, 'linear')
regtools.overlay_images(static, warped_moving, 'Static','Overlay','Warped moving',
'direct_warp_result.png')
```

And we can also apply the inverse mapping to verify that the warped static image is similar to the moving image

```
warped_static = mapping.transform_inverse(static, 'linear')
regtools.overlay_images(warped_static, moving,'Warped static','Overlay','Moving',
'inverse_warp_result.png')
```

Now let’s register a couple of slices from a b0 image using the Cross Correlation metric. Also, let’s inspect the evolution of the registration. To do this we will define a function that will be called by the registration object at each stage of the optimization process. We will draw the current warped images after finishing each resolution.

```
def callback_CC(sdr, status):
#Status indicates at which stage of the optimization we currently are
#For now, we will only react at the end of each resolution of the scale
#space
if status == imwarp.RegistrationStages.SCALE_END:
#get the current images from the metric
wmoving = sdr.metric.moving_image
wstatic = sdr.metric.static_image
#draw the images on top of each other with different colors
regtools.overlay_images(wmoving, wstatic, 'Warped moving', 'Overlay', 'Warped static')
```

Now we are ready to configure and run the registration. First load the data

```
from dipy.data.fetcher import fetch_syn_data, read_syn_data
from dipy.segment.mask import median_otsu
fetch_syn_data()
t1, b0 = read_syn_data()
data = np.array(b0.get_data(), dtype=np.float64)
```

We first remove the skull from the b0 volume

```
b0_mask, mask = median_otsu(data, median_radius=4, numpass=4)
```

And select two slices to try the 2D registration

```
static = b0_mask[:, :, 40]
moving = b0_mask[:, :, 38]
```

After loading the data, we instantiate the Cross Correlation metric. The metric receives three parameters: the dimension of the input images, the standard deviation of the Gaussian Kernel to be used to regularize the gradient and the radius of the window to be used for evaluating the local normalized cross correlation.

```
sigma_diff = 3.0
radius = 4
metric = CCMetric(2, sigma_diff, radius)
```

Let’s use a scale space of 3 levels

```
level_iters = [100, 50, 25]
sdr = SymmetricDiffeomorphicRegistration(metric, level_iters)
sdr.callback = callback_CC
```

And execute the optimization

```
mapping = sdr.optimize(static, moving)
warped = mapping.transform(moving)
```

We can see the effect of the warping by switching between the images before and after registration

```
regtools.overlay_images(static, moving, 'Static', 'Overlay', 'Moving',
't1_slices_input.png')
```

```
regtools.overlay_images(static, warped, 'Static', 'Overlay', 'Warped moving',
't1_slices_res.png')
```

And we can apply the inverse warping too

```
inv_warped = mapping.transform_inverse(static)
regtools.overlay_images(inv_warped, moving, 'Warped static', 'Overlay', 'moving',
't1_slices_res2.png')
```

Finally, let’s see the deformation

```
regtools.plot_2d_diffeomorphic_map(mapping, 5, 'diffeomorphic_map_b0s.png')
```

- Avants09
Avants, B. B., Epstein, C. L., Grossman, M., & Gee, J. C. (2009). Symmetric Diffeomorphic Image Registration with Cross-Correlation: Evaluating Automated Labeling of Elderly and Neurodegenerative Brain, 12(1), 26-41.

- Avants11
Avants, B. B., Tustison, N., & Song, G. (2011). Advanced Normalization Tools (ANTS), 1-35.

Example source code

You can download `the full source code of this example`

. This same script is also included in the dipy source distribution under the `doc/examples/`

directory.