This example shows how to use RUMBA-SD to reconstruct fiber orientation density functions (fODFs). This model was introduced by Canales-Rodriguez et al [CanalesRodriguez2015].

RUMBA-SD uses a priori information about the fiber response function (axial and perpendicular diffusivities) to generate a convolution kernel mapping the fODFs on a sphere to the recorded data. The fODFs are then estimated using an iterative, maximum likelihood estimation algorithm adapted from Richardson-Lucy (RL) deconvolution [Richardson1972]. Specifically, the RL algorithm assumes Gaussian noise, while RUMBA assumes Rician/Noncentral Chi noise – these more accurately reflect the noise generated by MRI scanners [Constantinides1997]. This algorithm also contains an optional compartment for estimating an isotropic volume fraction to account for partial volume effects. RUMBA-SD works with single- and multi-shell data, as well as data recorded in Cartesian or spherical coordinate systems.

The result from RUMBA-SD can be smoothed by applying total variation spatial regularization (termed RUMBA-SD + TV), a technique which promotes a more coherent estimate of the fODFs across neighboring voxels [Rudin1992]. This regularization ability is also included in this implementation.

- This example will showcase how to:
Estimate the fiber response function

Reconstruct the fODFs voxel-wise or globally with TV regularization

Visualize fODF maps

To begin, we will load the data, consisting of 10 b0s and 150 non-b0s with a b-value of 2000.

```
import numpy as np
from dipy.core.gradients import gradient_table
from dipy.data import get_fnames, get_sphere
from dipy.io.gradients import read_bvals_bvecs
from dipy.io.image import load_nifti
hardi_fname, hardi_bval_fname, hardi_bvec_fname = get_fnames('stanford_hardi')
data, affine = load_nifti(hardi_fname)
bvals, bvecs = read_bvals_bvecs(hardi_bval_fname, hardi_bvec_fname)
gtab = gradient_table(bvals, bvecs)
sphere = get_sphere('symmetric362')
```

There are multiple ways to estimate the fiber response function.

**Strategy 1: use default values**
One simple approach is to use the values included as the default arguments in
the RumbaSDModel constructor. The white matter response, wm_response has three
values corresponding to the tensor eigenvalues (1.7e-3, 0.2e-3, 0.2e-3). The
model has compartments for cerebrospinal fluid (CSF) (csf_response) and
grey matter (GM) (gm_response) as well, with these mean diffusivities set
to 3.0e-3 and 0.8e-3 respectively [CanalesRodriguez2015]. These default
values will often be adequate as RUMBA-SD is robust against impulse response
imprecision [Dell’Acqua2007]_.

```
from dipy.reconst.rumba import RumbaSDModel
rumba = RumbaSDModel(gtab)
print(f"wm_response: {rumba.wm_response}, " +
f"csf_response: {rumba.csf_response}, " +
f"gm_response: {rumba.gm_response}")
```

wm_response: [0.0017 0.0002 0.0002] csf_response: 0.003 gm_response: 0.0008

We can visualize what this default response looks like.

```
from dipy.sims.voxel import single_tensor_odf
from dipy.viz import window, actor
# Enables/disables interactive visualization
interactive = False
scene = window.Scene()
evals = rumba.wm_response
evecs = np.array([[0, 1, 0], [0, 0, 1], [1, 0, 0]]).T
response_odf = single_tensor_odf(sphere.vertices, evals, evecs)
# Transform our data from 1D to 4D
response_odf = response_odf[None, None, None, :]
response_actor = actor.odf_slicer(response_odf, sphere=sphere,
colormap='plasma')
scene.add(response_actor)
print('Saving illustration as default_response.png')
window.record(scene, out_path='default_response.png', size=(200, 200))
if interactive:
window.show(scene)
```

```
scene.rm(response_actor)
```

**Strategy 2: estimate from local brain region**
The csdeconv module contains functions for estimating this response.
auto_response_sst extracts an ROI in the center of the brain and isolates
single fiber populations from the corpus callosum using an FA mask with a
threshold of 0.7. These voxels are used to estimate the response function.

```
from dipy.reconst.csdeconv import auto_response_ssst
response, _ = auto_response_ssst(gtab, data, roi_radii=10, fa_thr=0.7)
print(response)
```

(array([0.00139919, 0.0003007 , 0.0003007]), 416.7372408293461)

This response contains the estimated eigenvalues in its first element, and the estimated S0 in the second. The eigenvalues are all we care about for using RUMBA-SD.

We can visualize this estimated response as well.

```
evals = response[0]
evecs = np.array([[0, 1, 0], [0, 0, 1], [1, 0, 0]]).T
response_odf = single_tensor_odf(sphere.vertices, evals, evecs)
# transform our data from 1D to 4D
response_odf = response_odf[None, None, None, :]
response_actor = actor.odf_slicer(response_odf, sphere=sphere,
colormap='plasma')
scene.add(response_actor)
print('Saving illustration as estimated_response.png')
window.record(scene, out_path='estimated_response.png', size=(200, 200))
if interactive:
window.show(scene)
```

```
scene.rm(response_actor)
```

**Strategy 3: recursive, data-driven estimation**
The other method for extracting a response function uses a recursive approach.
Here, we initialize a “fat” response function, which is used in CSD. From this
deconvolution, the voxels with one peak are extracted and their data is
averaged to get a new response function. This is repeated iteratively until
convergence [Tax2014].

To shorten computation time, a mask can be estimated for the data.

```
from dipy.reconst.csdeconv import recursive_response
from dipy.segment.mask import median_otsu
b0_mask, mask = median_otsu(data, median_radius=2,
numpass=1, vol_idx=np.arange(10))
rec_response = recursive_response(gtab, data, mask=mask, sh_order=8,
peak_thr=0.01, init_fa=0.08,
init_trace=0.0021, iter=4, convergence=0.001,
parallel=True, num_processes=2)
```

We can now visualize this response, which will look like a pancake.

```
rec_response_signal = rec_response.on_sphere(sphere)
# transform our data from 1D to 4D
rec_response_signal = rec_response_signal[None, None, None, :]
response_actor = actor.odf_slicer(rec_response_signal, sphere=sphere,
colormap='plasma')
scene.add(response_actor)
print('Saving illustration as recursive_response.png')
window.record(scene, out_path='recursive_response.png', size=(200, 200))
if interactive:
window.show(scene)
```

```
scene.rm(response_actor)
```

We will now use the estimated response function with the RUMBA-SD model to reconstruct the fODFs. We will use the default value for csf_response and gm_response. If one doesn’t wish to fit these compartments, one can specify either argument as None. This will result in the corresponding volume fraction map being all zeroes. The GM compartment can only be accurately estimated with at least 3-shell data. With less shells, it is recommended to only keep the compartment for CSF. Since this data is single-shell, we will only compute the CSF compartment.

RUMBA-SD can fit the data voxelwise or globally. By default, a voxelwise approach is used (voxelwise is set to True). However, by setting voxelwise to false, the whole brain can be fit at once. In this global setting, one can specify the use of TV regularization with use_tv, and the model can log updates on its progress and estimated signal-to-noise ratios by setting verbose to True. By default, both use_tv and verbose are set to False as they have no bearing on the voxelwise fit.

When constructing the RUMBA-SD model, one can also specify n_iter, recon_type, n_coils, R, and sphere. n_iter is the number of iterations for the iterative estimation, and the default value of 600 should be suitable for most applications. recon_type is the technique used by the MRI scanner to reconstruct the MRI signal, and should be either ‘smf’ for ‘spatial matched filter’, or ‘sos’ for ‘sum-of-squares’; ‘smf’ is a common choice and is the default, but the specifications of the MRI scanner used to collect the data should be checked. If ‘sos’ is used, then it’s important to specify n_coils, which is the number of coils in the MRI scanner. With ‘smf’, this isn’t important and the default argument of 1 can be used. R is the acceleration factor of the MRI scanner, which is termed the iPAT factor for SIEMENS, the ASSET factor for GE, or the SENSE factor for PHILIPS. 1 is a common choice, and is the default for the model. This is only important when using TV regularization, which will be covered later in the tutorial. Finally, sphere specifies the sphere on which to construct the fODF. The default is ‘repulsion724’ sphere, but this tutorial will use symmetric362.

```
rumba = RumbaSDModel(
gtab, wm_response=response[0], gm_response=None, sphere=sphere)
```

For efficiency, we will only fit a small part of the data. This is the same portion of data used in Reconstruction with Constrained Spherical Deconvolution.

```
data_small = data[20:50, 55:85, 38:39]
```

**Option 1: voxel-wise fit**
This is the default approach for generating ODFs, wherein each voxel is fit
sequentially.

We will estimate the fODFs using the ‘symmetric362’ sphere. This will take about a minute to compute.

```
rumba_fit = rumba.fit(data_small)
odf = rumba_fit.odf()
```

The inclusion of RUMBA-SD’s CSF compartment means we can also extract the isotropic volume fraction map as well as the white matter volume fraction map (the fODF sum at each voxel). These values are normalized such that they sum to 1. If neither isotropic compartment is included, then the isotropic volume fraction map will all be zeroes.

```
f_iso = rumba_fit.f_iso
f_wm = rumba_fit.f_wm
```

We can visualize these maps using adjacent heatmaps.

```
import matplotlib.pyplot as plt
fig, axs = plt.subplots(1, 2, figsize=(12, 5))
ax0 = axs[0].imshow(f_wm[..., 0].T, origin='lower')
axs[0].set_title("Voxelwise White Matter Volume Fraction")
ax1 = axs[1].imshow(f_iso[..., 0].T, origin='lower')
axs[1].set_title("Voxelwise Isotropic Volume Fraction")
plt.colorbar(ax0, ax=axs[0])
plt.colorbar(ax1, ax=axs[1])
plt.savefig('wm_iso_partition.png')
```

To visualize the fODFs, it’s recommended to combine the fODF and the isotropic components. This is done using the RumbaFit object’s method combined_odf_iso. To reach a proper scale for visualization, the argument norm=True is used in FURY’s odf_slicer method.

```
combined = rumba_fit.combined_odf_iso
fodf_spheres = actor.odf_slicer(
combined, sphere=sphere, norm=True, scale=0.5, colormap=None)
scene.add(fodf_spheres)
print('Saving illustration as rumba_odfs.png')
window.record(scene, out_path='rumba_odfs.png', size=(600, 600))
if interactive:
window.show(scene)
```

```
scene.rm(fodf_spheres)
```

We can extract the peaks from these fODFs using peaks_from_model. This will reconstruct the fODFs again, so will take about a minute to run.

```
from dipy.direction import peaks_from_model
rumba_peaks = peaks_from_model(model=rumba,
data=data_small,
sphere=sphere,
relative_peak_threshold=.5,
min_separation_angle=25,
normalize_peaks=False,
parallel=True,
num_processes=4)
```

For visualization, we scale up the peak values.

```
peak_values = np.clip(rumba_peaks.peak_values * 15, 0, 1)
peak_dirs = rumba_peaks.peak_dirs
fodf_peaks = actor.peak_slicer(peak_dirs, peak_values)
scene.add(fodf_peaks)
print('Saving illustration as rumba_peaks.png')
window.record(scene, out_path='rumba_peaks.png', size=(600, 600))
if interactive:
window.show(scene)
```

```
scene.rm(fodf_peaks)
```

**Option 2: global fit**
Instead of the voxel-wise fit, RUMBA also comes with an implementation of
global fitting where all voxels are fit simultaneously. This comes with some
potential benefits such as:

More efficient fitting due to matrix parallelization, in exchange for larger demands on RAM (>= 16 GB should be sufficient)

The option for spatial regularization; specifically, TV regularization is built into the fitting function (RUMBA-SD + TV)

This is done by setting voxelwise to False, and setting use_tv to True.

TV regularization requires a volume without any singleton dimensions, so we’ll have to start by expanding our data slice.

- ::
- rumba = RumbaSDModel(gtab, wm_response=response[0], gm_response=None,
voxelwise=False, use_tv=True, sphere=sphere)

data_tv = data[20:50, 55:85, 38:40]

Now, we fit the model in the same way. This will take about 90 seconds.

```
rumba_fit = rumba.fit(data_tv)
odf = rumba_fit.odf()
combined = rumba_fit.combined_odf_iso
```

Now we can visualize the combined fODF map as before.

```
fodf_spheres = actor.odf_slicer(combined, sphere=sphere, norm=True,
scale=0.5, colormap=None)
scene.add(fodf_spheres)
print('Saving illustration as rumba_global_odfs.png')
window.record(scene, out_path='rumba_global_odfs.png', size=(600, 600))
if interactive:
window.show(scene)
```

This can be compared with the result without TV regularization, and one can observe that the coherence between neighboring voxels is improved.

```
scene.rm(fodf_spheres)
```

For peak detection, peaks_from_model cannot be used as it doesn’t support global fitting approaches. Instead, we’ll compute our peaks using a for loop.

```
from dipy.direction import peak_directions
shape = odf.shape[:3]
npeaks = 5 # maximum number of peaks returned for a given voxel
peak_dirs = np.zeros((shape + (npeaks, 3)))
peak_values = np.zeros((shape + (npeaks,)))
for idx in np.ndindex(shape): # iterate through each voxel
# Get peaks of odf
direction, pk, _ = peak_directions(odf[idx], sphere,
relative_peak_threshold=0.5,
min_separation_angle=25)
# Calculate peak metrics
if pk.shape[0] != 0:
n = min(npeaks, pk.shape[0])
peak_dirs[idx][:n] = direction[:n]
peak_values[idx][:n] = pk[:n]
# Scale up for visualization
peak_values = np.clip(peak_values * 15, 0, 1)
fodf_peaks = actor.peak_slicer(peak_dirs[:, :, 0:1, :],
peak_values[:, :, 0:1, :])
scene.add(fodf_peaks)
print('Saving illustration as rumba_global_peaks.png')
window.record(scene, out_path='rumba_global_peaks.png', size=(600, 600))
if interactive:
window.show(scene)
```

```
scene.rm(fodf_peaks)
```

- CanalesRodriguez2015(1,2)
Canales-Rodríguez, E. J., Daducci, A., Sotiropoulos, S. N., Caruyer, E., Aja-Fernández, S., Radua, J., Mendizabal, J. M. Y., Iturria-Medina, Y., Melie-García, L., Alemán-Gómez, Y., Thiran, J.-P., Sarró, S., Pomarol-Clotet, E., & Salvador, R. (2015). Spherical Deconvolution of Multichannel Diffusion MRI Data with Non-Gaussian Noise Models and Spatial Regularization. PLOS ONE, 10(10), e0138910. https://doi.org/10.1371/journal.pone.0138910

- Richardson1972
Richardson, W. H. (1972). Bayesian-Based Iterative Method of Image Restoration*. JOSA, 62(1), 55–59. https://doi.org/10.1364/JOSA.62.000055

- Constantinides1997
Constantinides, C. D., Atalar, E., & McVeigh, E. R. (1997). Signal-to-Noise Measurements in Magnitude Images from NMR Phased Arrays. Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 38(5), 852–857.

- Rudin1992
Rudin, L. I., Osher, S., & Fatemi, E. (1992). Nonlinear total variation based noise removal algorithms. Physica D: Nonlinear Phenomena, 60(1), 259–268. https://doi.org/10.1016/0167-2789(92)90242-F

- DellAcqua2007
Dell’Acqua, F., Rizzo, G., Scifo, P., Clarke, R., Scotti, G., & Fazio, F. (2007). A Model-Based Deconvolution Approach to Solve Fiber Crossing in Diffusion-Weighted MR Imaging. IEEE Transactions on Bio-Medical Engineering, 54, 462–472. https://doi.org/10.1109/TBME.2006.888830

- Tax2014
Tax, C. M. W., Jeurissen, B., Vos, S. B., Viergever, M. A., & Leemans, A. (2014). Recursive calibration of the fiber response function for spherical deconvolution of diffusion MRI data. NeuroImage, 86, 67–80. https://doi.org/10.1016/j.neuroimage.2013.07.067

Example source code

You can download `the full source code of this example`

. This same script is also included in the dipy source distribution under the `doc/examples/`

directory.